BCR::ABL1 tyrosine kinase domain (TKD) variant detection moved to Next Generation Sequencing

Sample requirements

Please send 15-20ml peripheral blood or 3-5ml bone marrow in EDTA that will be receipted within the SIHMDS laboratory <72 hours from collection. In instances where this is not possible, please send >25ul of cDNA.

Test requests

Please use the NT-GLH Specialist HaemOnc request form and ensure the following information is provided:

• Diagnosis (e.g. CML [M84.8], AML [M80.15], ALL [M91.11] or AL other [M89.17]).
• BCR::ABL1 transcript type.
• Current therapy.
• Reason for referral.
• Address for report.
• Requesting clinician.
• Patient demographics.

Please note that testing will only be performed on samples with a BCR::ABL1 IS ratio >0.1%.

Laboratory address:
Samples need to be sent to:
SIHMDS,
Imperial College Healthcare NHS Trust,
G-Block, Level 2,
Hammersmith Hospital,
Du Cane Road, London, W12 0HS

The turnaround time for this test is 21 calendar days. Please note that this is currently awaiting full UKAS accreditation.

Please contact the Imperial SIHMDS Team or get in touch with the GLH team if you have any queries regarding this update.

SIHMDS Imperial (molecular)
imperial.moleculardiagnostics@nhs.net
Tel: 020 3313 2179

Chloe Hayden
Principal Clinical Scientist
SIHMDS NWLP, Imperial College Healthcare NHS Trust
chloe.hayden@nhs.net

Dörte Wren
Lead Scientist for Cancer Genomics
North Thames GLH
Dorte.Wren@gosh.nhs.uk