Changes to MLH1 promotor hypermethylation/MSI testing – Important Information for Service Users

Please find below information about how to request this test below, including a new request form.

Sample requirements:

Referrals for MLH1 promotor hypermethylation/MSI testing require 5 x 10 µm mounted FFPE sections (unstained and uncharged). Please ensure that the tumour cell content is provided and an H&E-stained section with areas of tumour clearly marked is send alongside the sample material.

Test requests:

Electronic test requesting and reporting is available through the RMH Order&View portal. For further details and to obtain access to the system please contact the O&V system administrator Henry Coleman (Henry.Coleman@rmh.nhs.uk / Admin.OrderView@rmh.nhs.uk).

Alternatively, samples can be send with a completed request form and reports will be returned by nhs.net. You can access the test request form here. Please provide the relevant contact information on the request form.

Laboratory address:

Samples need to be send to:

Clinical Genomics Department
The Centre for Molecular Pathology
The Royal Marsden NHS Foundation Trust
15, Cotswold Road, Sutton, Surrey, SM2 5NG

MLH1 Promotor Hypermethylation testing is available for known cases of colorectal cancer and endometrial cancer for the evaluation of Lynch syndrome.

Please refer to the NHSE guidance for testing eligibility which can be found in the test directory under R210 (https://www.england.nhs.uk/publication/national-genomic-test-directories/) and also see the relevant NICE guidance algorithms (https://www.nice.org.uk/guidance/dg27 and https://www.nice.org.uk/guidance/dg42).

Please contact the Clinical Genomics lab or get in touch with the GLH team if you have any queries regarding this update.

Clinical Genomics Laboratory (The Royal Marsden)
rmh-tr.moleculardiagnostics@nhs.net
Tel: 020 8925 6565

Mikel Valganon Petrizan
RMH Head of Clinical Genomics
Mikel.valganonpetrizan@rmh.nhs.uk

Dörte Wren
Lead Scientist for Cancer Genomics, North Thames GLH
Dorte.wren@gosh.nhs.uk